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Detalles de la obra

Título:
Phage display
Subtítulo:
a laboratory manual
Autor(es):
Barbas, Carlos F.; Burton, Dennis R.; Scott, Jamie K.; Silverman, Gregg J.
Pie de imprenta:
Nueva York: Cold Spring Harbor Laboratory Press, (c)2001
Descripción física:
[280] p. diagrs., grafs. 26 x 18 cm
Idioma:
Inglés
ISBN:
978-087969740-2
Resumen:
Contenido: 1. Filamentous phage biology / R. Webster - 2. Phage-display vectors / J. K. Scott, C. F. Barbas - 3. Antibody libraries / D. R. Burton - 4. Peptide libraries / J. K. Scott - 5. Functional domains and scaffolds / C. J. Silverman - 6. Gene fragment libraries and genomic and cDNA expression cloning / R. A. Williamson, G. J. Silverman - 7. Overview: Amplification of antibody genes / D. R. Burton - 8. Generation of antibody libraries: Immunization, RNA Preparation, and cDNA Synthesis / J. Andris-Widhopf, C. Rader, C. F. Barbas - 9. Generation of antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences / J. Andris-Widhopf, P. Steinberger, R. Fuller, C. Rader, C. F. Barbas - 10. Selection from antibody libraries / C. Rader, P. Steinberger, C.F. Barbas - 11. Analysis of selected antibodies / P. Steinberger, C. Rader, C.F. Barbas - 12. Production and purfification of Fab and scFv / M. Elia, J. Andris-Widhopf, R. Fuller, C. F. Barbas - 13. Antibody engineering / C. Rader, C.F. Barbas - 14. Overview : Peptide libraries / J. K. Scott - 15. General phage methods / L. L. C. Bonnycastle, A. Menendez, J. K. Scott - 16. Production peptide libraries / L. L. C. Bonnycastle, J. Shen, A. Menendez, J. K. Scott - 17. Screening peptide libraries / A. Menendez, L. L. C. Bonnycastle, O. C. C. Pan, J. K. Scott - 18. Analysis of phage-borne peptides / M.B. Zwick, A. Menendez, L. L. C. Bonnycastle, J. K. Scott - 19. Construction and use of pIII-displayed peptide libraries / K. A. Noren, L. H. Saltman, C. J. Noren - 20. Construction and selection from gene fragment phage-display expression libraries / G. J. Silverman - 21. Construction and selection from cDNA phage-display expression libraries / G. J. Silverman - 22. In vivo selection of phage-display libraries / R. Pasqualini, W. Arap, D. Rajotte, E. Ruoslahti - 23. Cell surface selection and analysis of monoclonal antibodies from phage libraries / D. L. Siegel.
Notas:
Incluye Indice analítico.
Ubicación física:
BI 579.26 / BAR
Tipo de material:
[Material Impreso]